Allergic Airway Diseases

Voraphani 2014

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Voraphani 2014

Basic info

  • GEO: GSE43696
  • reference: [Voraphani14]
  • platform: Agilent Human GE 4x44K V2
  • tissue: bronch biopsy
  • tissue environment: ex-vivo

Note

The asthma dx was specified as severe and moderate so all non-control dxs were set to asthma.

Samples

asthma AR controls total
66 0 25 91

The following is a summary of the covariate information extracted from downloaded GSE files.

Gene expression analysis

Tue Aug 09 08:47:20 AM 2016
total files: 1
file: 1/1
file name: GSE43696_series_matrix.txt.gz
status: Public on Jul 01 2014 
data processing: Agilent Feature Extraction v10.7.3.1 default parameters 
type: RNA 
probes 41000 
samples 108 
platform_id: GPL6480 
covariates extracted from eset...
	disease_state
	cell_type
	gender
	age
model: ~0+dx+age+gender 
gene filter 30936/41000
There were 268 significant probes

Heatmaps

heatmap

Outliers removed

heatmap

Heatmaps of the top 50 genes. The genes are clustered based on normalized values of intensity. The quality control check is that the samples cluster according to phenotype.

Outlier analysis

pca
pca

Expression results

limma plots

Outliers removed

limma plots

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